Direct Diagnosis of Echovirus 12 Meningitis Using Metagenomic Next Generation Sequencing
نویسندگان
چکیده
منابع مشابه
Assessment of Metagenomic Assembly Using Simulated Next Generation Sequencing Data
Due to the complexity of the protocols and a limited knowledge of the nature of microbial communities, simulating metagenomic sequences plays an important role in testing the performance of existing tools and data analysis methods with metagenomic data. We developed metagenomic read simulators with platform-specific (Sanger, pyrosequencing, Illumina) base-error models, and simulated metagenomes...
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Abstract DNA sequencing is one of the great valuable techniques in molecular biology, which can be used to detect the sequence of nucleotides in a DNA fragment. The high-throughput sequencing known as Next Generation Sequencing (NGS) revolutionized genomic research and molecular biology; therefore, the whole human genome can be sequenced with a low cost in several days. NGS technology is simi...
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A diagnosis of brucellosis can be difficult because routine culture and serological methods exhibit variable sensitivity and specificity. We present the use of a metagenomic next- generation sequencing assay to diagnose a case of neurobrucellosis from cerebrospinal fluid, resulting in the institution of appropriate antibiotic treatment and a favorable clinical outcome.
متن کاملStrategies and Clinical Applications of Next Generation Sequencing
Abstract DNA sequencing is one of the great valuable techniques in molecular biology, which can be used to detect the sequence of nucleotides in a DNA fragment. The high-throughput sequencing known as Next Generation Sequencing (NGS) revolutionized genomic research and molecular biology; therefore, the whole human genome can be sequenced with a low cost in several days. NGS technology is simi...
متن کاملCorrection: Assessment of Metagenomic Assembly Using Simulated Next Generation Sequencing Data
The first sentence of the second paragraph of the ‘‘Assemblies’’ subsection of the Methods should have cited reference 34 instead of 33. The correct sentence should read: The Illumina datasets were assembled using SOAPdenovo 1.05 [34] using following parameters: ‘‘-K 23 -L 70 -M 3 -u -R -F’’. The fourth sentence of the first paragraph of the ‘‘Importance of Quality Control for Illumina Data’’ s...
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ژورنال
عنوان ژورنال: Pathogens
سال: 2021
ISSN: 2076-0817
DOI: 10.3390/pathogens10050610